Home > Sandwich ELISA Kits > Rat Serine/threonine-protein kinase mTOR (mTOR) ELISA Kit

Product Name:

Rat Serine/threonine-protein kinase mTOR (mTOR) ELISA Kit

Cat.#:

JL45835

Regulatory Status:

RUO

Alternative:

FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; FK506 Binding Protein 12 Rapamycin Associated Protein; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.1ng/mL

Specificity:

This kit recognizes Rat mTOR in samples. No significant cross-reactivity or interference between Rat mTOR and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| mTOR

The mammalian target of rapamycin (mTOR),also referred to as the mechanistic target of rapamycin, and sometimes called FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that is encoded by the MTOR gene.mTOR is a member of the phosphatidylinositol 3-kinase-related kinase family of protein kinases.mTOR links with other proteins and serves as a core component of two distinct protein complexes, mTOR complex 1 and mTOR complex 2, which regulate different cellular processes.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.6	1.74	1.07	0.66	0.43	0.31	0.23	0.09
Corrected OD	2.51	1.65	0.98	0.57	0.34	0.22	0.14	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat mTOR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	92-105	102
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Rat mTOR and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	88-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	108